Hom–Kularb–Dang Rice Bran Extract for the Prevention of UVB-Damage Against Human Skin Fibroblast

Abstract

In this study, we investigated the protective effect of hydroglycolic crude extract of Thai red Hom–Kularb–Drice (HKD) rice bran on UVBinduced photoaging of human skin fibroblast. The HKD rice bran extract showed scavenging capacity with an EC50 of 4.37± 0.34 mg/mL testing using a DPPH radical scavenging assay. The structural identification of proanthocyanidins compounds was performed by Liquid chromatographyelectrospray ionization- quadrupole-time- of flight mass spectrometry (LCESI-Q-TOF-MS) in the hydroglycolic HKD extract. These compounds were cyanidin B2 type and ( epi) catechin. The extract showed a no cytotoxic response was showed in human skin fibroblast. Furthermore, the HKD extract ( 20 µg/mL) exhibited a protective effect in UVB irradiated primary skin fibroblast, reducing MMP-1 (matrix metalloproteinases- 1) expression and increasing production of type I procollagen. This data suggests that the HKD rice bran extract has potential for use as a skin anti- aging agent in cosmetic products.